A novel machine learning-based approach for the detection and analysis of spontaneous synaptic currents.

A novel machine learning-based approach for the detection and analysis of spontaneous synaptic currents.

Blog Article

Spontaneous synaptic activity is a hallmark of biological neural networks.A thorough description of these synaptic signals is essential for understanding neurotransmitter release and the generation of a High Rise Short postsynaptic response.However, the complexity of synaptic current trajectories has either precluded an in-depth analysis or it has forced human observers to resort to manual or semi-automated approaches based on subjective amplitude and area threshold settings.

Both procedures are time-consuming, error-prone and likely affected by human bias.Here, we present three complimentary methods for a fully automated analysis of spontaneous excitatory postsynaptic currents measured in major cell types of the mouse retina and in a primary culture of mouse auditory Basketball cortex.Two approaches rely on classical threshold methods, while the third represents a novel machine learning-based algorithm.

Comparison with frequently used existing methods demonstrates the suitability of our algorithms for an unbiased and efficient analysis of synaptic signals in the central nervous system.